Sartorius Claristep filtration system
Preparing samples by clarification is an essential step prior to nearly all analytical techniques, such as high-pressure liquid chromatography (HPLC). This filtration step to eliminate particles is crucial for maintaining the integrity of chromatography columns and for maximising their operating lifetime. In addition, as the sensitivity of automated analytical instruments continues to improve, they increasingly require less volume to operate in order to maximise throughput.
Fast small-volume clarification that does not add leachables or extractables to the original sample is important for achieving the best analytical results. To meet these requirements, Sartorius has developed an easy-to-use and straightforward filtration set-up. The manually operated Claristep filtration system consisting of a station and filter units offers a novel way for clarifying samples prior to analysis.
The station consists of a base, a lid and an exchangeable tray for easy and accurate positioning of sample vials and Claristep filter units. The design features grooves in the station’s lid and matching guide ridges on the filter units to enable intuitively correct alignment and convenient handling of the system. The grooves automatically guide the filter unit caps into the correct positions for simultaneous and accurate cap closure.
The system enables up to eight samples to be simultaneously filtered — without needing any power supply or a vacuum/pressure source. Single-use filter units with RC membranes are designed for sample volumes of 60 to 600 µL, with a hold-up volume of less than 30 µL. The units have been optimised for solvents and aqueous solutions and provide chemical compatibility as well as low non-specific binding of analytes.
The contact time of the samples with the filters and the caps is short, ensuring contamination-free results. Filtrates are collected in any 12 x 32 mm outer diameter vials of the user’s choice based on the analytical method to be performed.
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