GeneCopoeia AAVPrime adeno-associated virus (AAV) particles
GeneCopoeia’s AAVPrime adeno-associated virus (AAV) products are useful tools for inserting genes into a broad range of cell types with high efficiency and enhanced safety. GeneCopoeia’s optimised helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made particles are available in 12 different serotypes, such as fluorescent reporters. Users can also request custom AAV particles for genes up to 3 kb in length.
Key advantages include: high titers — a titer of purified particles can be up to 1014 GC/mL; high adaptability — all serotypes are available; versatility, making the product usable in a broad range of host cell types; low toxicity, as the product does not integrate into the host genome; low immunogenicity, with minimal host immune response; and safety, as the product is not associated with any human disease.
AAVPrime uses a helper virus-free system for the safe preparation of high-titer AAV particles. HEK293T cells are co-transfected with three plasmids encoding the factors necessary for recombinant AAV packaging and purified AAV particles are either membrane purified or purified by two-phase partitioning for high-titer AAV ready for in vivo animal use.
The destination vector carries the user’s gene of interest (GOI) and two ITRs. Users can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection. The plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.
For more information: http://www.genecopoeia.com/product/aav/aavprime-adeno-associated-virus-particles/.
Phone: 02 4575 0309
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