Dolomite Bio Injection Valve and Sample Loop for single-cell RNA sequencing

Thursday, 12 January, 2017 | Supplied by: John Morris Scientific Pty Ltd


Dolomite Bio has launched an Injection Valve and Sample Loop for single-cell RNA sequencing workflows. This option enables straightforward, gentle introduction of evenly distributed mRNA capture beads into the company’s RNA-Seq System for individual encapsulation of cells.

The Injection Valve and Sample Loop is a complete module allowing barcoded mRNA capture beads in lysis buffer to be efficiently flowed through the RNA-Seq Chip without clustering or blockages. Bead damage prior to encapsulation is negligible as harsh mechanical stirring is unnecessary, and its four-way valve and simple, compact microfluidic connections enable seamless switching between the injection of the bead suspension and pumping of a driving fluid, with minimal dead volume and bead wastage compared to traditional syringe pump methods.

The integrated mirror and chip holder enable easy visualisation via the RNA-Seq System’s high-speed microscope and camera, and the module also incorporates a Falcon/Eppendorf tube holder for easy collection of the droplet emulsion.

Online: www.johnmorris.com.au
Phone: 02 9496 4200
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