Due to the heterogeneous nature of histological specimens, it is necessary to isolate specific single cells or cell groups from the natural tissue complex before they can be subjected to PCR analysis. Laser microdissection is a convenient, contamination-free step in the preparation of RNA, DNA and protein analysis.
The Leica Laser Microdissection System is based on an upright automated microscope, where all the functions, such as laser activation, cutting movement, specimen movement, magnification change and focusing are motorised, and controlled by a PC. The tray collecting the dissected material is also positioned by PC control for an efficient workflow and clear assignment of specimen and dissected cells in the PCR tube. The microscope image is recorded and displayed on the PC monitor.
Small areas of tissue - even single cells - are simply outlined on the PC image, then a focused beam from a pulsed UV laser is directed along the contours of the areas of interest. The areas cut out by the laser are transferred to the PCR tubes purely by the force of gravity. This technology ensures gentle treatment of the specimen, particularly as the specimen is not heated, and eliminates the risk of specimen contamination as mechanical contact is avoided.
