The Center for Experimental Bioinformatics (CEBI) at the University of Southern Denmark is using Thermo Scientific nano-ultra high performance liquid chromatography (UHPLC) technology to accelerate productivity and improve efficiencies during its proteomics research. Implementation of the Thermo Scientific EASY-nLC 1000 has enabled 34% more peptide and 22% more protein identifications within the CEBI laboratory. Additionally, the instrument has improved separations and facilitated the analysis of more complex samples within the laboratory.
CEBI is a specialist proteomics research laboratory, aimed at applying modern methods of mass spectrometry and proteomics to functional analysis of genes. Prior to implementing nano-UHPLC technology, CEBI used conventional HPLC. However, it needed a more complete solution to accelerate productivity and improve efficiencies. The EASY-nLC 1000 is used in conjunction with Thermo Scientific ion trap and Orbitrap mass spectrometers for a wide range of proteomics research, including the study of complex mouse dendritic cells.
The device has allowed CEBI to take advantage of the unique ability to facilitate dedicated separation of biomolecules at ultra-high pressures. The higher operating pressures provided by the system have increased the number of peptide and protein identifications performed within the laboratory.
The instrument also allows selection of the most appropriate column dimensions and solid phase materials in order to identify the most suitable configuration to match analytical requirements. Using thinner, longer columns and smaller beads has improved separation and ionisation, enhancing sensitivity and allowing the CEBI team to scale down experiments and reduce costs.