GeneCopoeia stable cell line development services

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GeneCopoeia offers services for establishing stable cell lines for protein overexpression, gene knockdown or genome editing which meet the user’s specific research needs.

Stable cell lines produced by the company can overexpress virtually any protein of interest in the user’s cell line of choice. Users can choose to have their own expression cassette integrated into the genome or from among more than 53,000 human and 32,000 mouse ORFs from the ORF clone collection.

Applications include monoclonal antibody production; protein production for biochemical assays; protein production for crystal or NMR structural determination; fusion tagging for live cell imaging; fusion tagging for pulldown/immunoprecipitation; drug target analysis.

GeneCopoeia will design shRNAs and integrate them into virtually any cell line. Applications include determination of gene function in cells; expression profiling to identify candidate downstream genes in a network or pathway; drug target validation.

The company also offers stable cell lines carrying specific, targeted modifications created with either TALEN or CRISPR-Cas9. They will design the genome editing tool target site, donor constructs used for homologous recombination-mediated applications, transfect the cell line of choice with the genome editing tools and isolate single or double allele-modified clones. The service can also be used with the human and mouse safe-harbour integration system or human safe-harbour knock-in ORF clones.

Applications include gene knockout; introduction of point mutations or defined insertions/deletions; correction of disease mutations back to wild type; transgene knock-in at a customer-specified locus or at human and mouse safe-harbour sites; gene tagging; promoter or gene replacement.