DNA & RNA

Article (5)
  • Nucleofection - combining high transfection performance with superior preservation of functionality
    Nucleofection has become a method of choice whenever transfection of primary cells or difficult-to-transfect cell lines is required. Here we show that Nucleofection of frequently used primary cells (mouse dendritic cells, human macrophages and human T cells) results in highly efficient transfer of DNA and other substrates, while at the same time maintaining excellent cell viability and post-transfection functionality. This combination of benefits makes Nucleofection superior to other transfection methods.
  • Quantitative RNA analysis with the FlashGel system for RNA
    Assessment of RNA quality/integrity is key to consistent performance in a variety of uses, including microarrays, RT-PCR, and RTqPCR. Chip-based analysis methods provide a fast, sensitive, and quantitative rating of RNA quality (RNA integrity number (RIN)). However, such systems tend to be less flexible and more expensive than traditional gel-based methods of RNA analysis.
  • Geometry rules RNA shapes
    It seems that the rules that dictate the three-dimensional shapes of RNA molecules are based simply on geometry and not on complex chemical interactions.
  • The DNA transistor
    In an effort to build a nanoscale DNA sequencer, IBM scientists are drilling nano-sized holes in computer-like chips and passing DNA strands through them in order to read the information contained within their genetic code.
  • RNA isolation within specific cells
    A team of University of Oregon biologists, using fruit flies, has created a way to isolate RNA from specific cells, opening a new window on how gene expression drives normal development and disease-causing breakdowns.
Feature (3)
  • First human DNA large-scale variation map is produced
    Researchers in the US have produced the first sequence-based map of large-scale structural variation across the human genome.
  • Discrimination and DNA databases
    Last month, the South Australian government began accessing the national DNA database. In just the first few days the database has linked suspects to more than 20 unsolved crimes, including rape and assault, some dating back to the 1980s
  • Who owns your DNA?
    There is no doubt that genetic testing and the mapping of the human genome has changed the face of medicine, but it also raises new debates over ethics and, more importantly, who owns your genetic information?
Product (39)
  • GeneCopoeia miTarget miRNA target sequence expression clones
    GeneCopoeia offers genome-wide microRNA 3′ UTR target constructs in mammalian expression vector systems with luciferase reporter genes for functional validation experiments.
  • Applied Biosystems SOLiD Total RNA-Seq Kit
    The SOLiD Total RNA-Seq Kit offers a streamlined solution for the construction of next-generation sequencing libraries for either whole transcriptome or small RNA analysis. The optimised kit enables hypothesis-neutral discovery of coding RNA, non-coding RNA, novel transcripts, alternate splicing, small RNAs and isoforms, while maintaining accurate sequence representation and preserving strand orientation.
  • Integrated DNA Technologies Zen double-quenched probe
    Integrated DNA Technologies has introduced a double-quenched probe that increases the accuracy and reliability of 5′ nuclease qPCR experiments. While traditional probes have 20-30 bases between the dye and quencher, this probe design positions an internal Zen quencher only 9 bases from the 5′ fluorophore.
  • Qiagen GeneGlobe web portal
    On the GeneGlobe web portal, users can explore and order gene and pathway specific products for a range of applications. For miRNA research, the miScript target protector is available for researchers doing functional analysis of miRNAs.
  • GenoLogics and CLC bio end-to-end next-generation sequencing
    GenoLogics and CLC bio have announced they will provide an end-to-end informatics and analysis solution, optimised for next-generation sequencing research, that addresses both laboratory and data management and data analysis, all within one integrated system.
  • Agilent Technologies SureSelect Target Enrichment System
    The Agilent Technologies SureSelect Target Enrichment System, based on a hybrid selection technique, can be scaled to meet the needs of larger sequencing projects.
  • AmpFISTR Identifiler Direct, Identifiler Plus and NGM PCR Amplification Kits
    Forensic DNA kits with chemistry reagents, the AmpFISTR Identifiler Direct, Identifiler Plus and NGM PCR Amplification Kits provide good performance, efficiency and data quality, enabling fast recovery of more DNA results from a wider range of samples.
  • Agilent SureSelect Human All Exon kit
    The SureSelect Human All Exon Kit for the Applied Biosystems SOLiD System enables target enrichment prior to next-generation DNA sequencing.
  • Gel documentation system
    Vilber Lourmat has announced the availability of its latest E-box, the VX-2, designed to occupy minimum bench space.
  • Microchip electrophoresis system
    Shimadzu’s MultiNA Microchip Electrophoresis System provides for fully automated DNA/RNA size confirmation and quantitation.
  • Genetic analysers
    Designed with ease of use in mind, the 3500 Series Genetic Analyzers operate on standard power outlets and have no need for heat-ducting infrastructure. With an increased throughput, faster run times, innovative reagents and intuitive software, the 3500 Series is suited to capillary sequencing and fragment analysis.
  • DNA kits
    The QIAxcel DNA kits by QIAGEN replaces traditional gel analysis of DNA and RNA. Researchers can perform DNA fragment analysis in as little as ten minutes for 12 samples without the need for agarose gel preparation.
  • Electroporation system cuvette
    Bio-Rad Laboratories has launched the Gene Pulser MXcell ShockPod cuvette chamber for its Gene Pulser MXcell electroporation system. The ShockPod offers researchers the flexibility to easily transition between electroporation plates and familiar cuvettes on the Gene Pulser MXcell electroporation system.
  • High-throughput magnetic DNA and RNA purification
    The Thermo Scientific KingFisher Flex is a compact magnetic bead processing system for rapid purification of DNA, RNA, proteins or cells. It can process 24 or 96 samples simultaneously and has a processing volume of 20-5000 µL. Additionally, the KingFisher Flex can be used with any brand of magnetic bead kit available as well as in-house developed kits.
  • Cell transfection
    The Invitrogen Neon Transfection Device is a benchtop tool that enables researchers to better understand the functions of cells, genes and proteins. This device simplifies the delivery of DNA, RNA and proteins into a wide range of mammalian cell types, especially difficult-to-transfect cells, such as many types of primary and stem cells.
  • miRNA array
    The GeneChip miRNA array from Affymetrix is a powerful tool for studying the role of microRNAs (miRNAs) and their importance in cancer and other diseases and the discovery of biomarkers and disease signatures.
  • RNA isolation kit
    The PureLink RNA Mini Kit provides a simple, reliable and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells and tissue, blood, bacteria, yeast and liquid samples.
  • Plasmid purification kit
    The GenElute HP 96-well plasmid miniprep kit for DNA purification is suitable for a variety of pharmaceutical and biotechnology research applications, providing rapid purification of plasmid DNA that can generate transfection-grade plasmid with high viral titers.
  • DNA/RNA purification system
    Phenomenex’s Clarity QSP system for synthetic RNA and DNA purification uses trityl-on purification to deliver concentrated, full-length oligonucleotide sequences.
  • Plasmid purification kit
    The GenElute HP 96-well plasmid miniprep kit for DNA purification is suitable for a variety of pharmaceutical and biotechnology research applications, providing rapid purification of plasmid DNA that can generate transfection-grade plasmid with high viral titers.
  • Standardised epigenetic kits
    Qiagen has launched five additions to its EpiTect product line to help address the growing demand for standardised products for complete workflows in epigenetics.
  • Cell particle processor
    The Thermo Scientific Kingfisher Flex is a compact magnetic bead particle processing system for purification of DNA, RNA, proteins or cells. It can process 96 samples simultaneously and has a processing volume of 20 to 5000 µL.
  • DNA transfer system
    The iBlot Transfer Device can be used for transferring DNA samples from agarose or polyacrylamide gels to nylon membranes in 7 minutes. It can be used to transfer protein from SDS-PAGE gels into nitrocellulose and PVDF membranes in 6 to 8 minutes, as compared with 90 minutes or more in conventional wet or semi-dry blotting.
  • Whole transcriptome amplification
    Sigma-Aldrich has launched the TransPlex Whole Transcriptome Amplification (WTA2) technology. TransPlex technology is said to be a fast, sensitive and robust method for amplifying total RNA from a variety of sample tissue sources such as animals, plants and microorganisms.
  • Nucleic acid extractor
    The Spri-te nucleic acid extractor for the automated purification of DNA and RNA prepares between one and 10 samples in about 30 minutes and requires minimal user training.
  • cDNA synthesis kit
    The SuperScript Vilo cDNA synthesis kit provides reliable and sensitive first strand cDNA synthesis. The kit uses RNAse H reduced SuperScript III Reverse Transcriptase, generating greatly increased yields of cDNA and improving qPCR sensitivity.
  • Microchip electrophoresis system
    The MCE-202 MultiNA quickly and easily performs DNA and RNA nucleic acid size confirmation and quantitation. It uses microchip technology to conduct fully automated high-speed electrophoresis separation, with fluorescence detection for high-sensitivity analysis.
  • DNA fragment cloning
    MultiSite Gateway Pro Technology enables users to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway Expression vector.
  • Nucleic acid extraction from blood
    Fuji Photo Film has entered the market for nucleic acid extraction with the QuickGene-810. It consists of a compact, space-saving tabletop device (40 x 45 cm) and matching kits for extraction of DNA and RNA. Using this system, DNA can be obtained from whole blood in just six minutes (for eight samples).
  • DNA separation
    Cambrex's FlashGel System can be used to separate DNA in 2-7 mins while monitoring migration in real time on the bench under normal laboratory light conditions (no harmful UV illumination).
  • Introducing Personal Automation
    The Maxwell 16 System offers automated and truly walk-away nucleic acid purification that saves time and labour by eliminating reagent preparation, pipetting and centrifugation steps.
  • Chromosome-specific paint system
    Star*FISH and Chromotrax paint systems are highly chromosome specific and provide good, even coverage of the chromosome, unlike paints based on cloned libraries. This is because they are prepared from DOP-PCR*-amplified DNA obtained from flow-sorted chromosomes.
  • Gel documentation system
    Syngene has introduced the GeneFlash USB, claimed to be the first gel documentation system on the market to use USB memory device technology to accurately record gel images that are both PC and Mac compatible.
  • DNA sequencing platform
    The Solid System DNA sequencing platform is claimed to enable new ways of performing genetic analysis applications.
  • DNA and protein gel stain
    GelRed is a red fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for gel staining.
  • Real-time transilluminator
    With the E-Gel Safe Imager Real-Time Transilluminator, users can watch migration of DNA bands in real time when carrying out electrophoresis using the E-Gel iBase and E-Gel Pre-Cast Agarose gel containing SYBR Safe DNA Gel Stain. The transilluminator is designed to fit beneath the E-Gel iBase Power System and is intended for use with E-Gels containing SYBR Safe. Blue light emitted by the instrument is suitable for viewing DNA that is stained with SYBR Safe.
  • Smarter DNA gel-purification
    The easiest way to gel extract your DNA band.
  • Micro RNA profiling
    Qiagen has added to its portfolio of miRNA technologies with the introduction of the miScript System, which enables detection and quantification of miRNA, as well as mRNA and other small RNA, using SYBR Green-based, real-time PCR.
  • Sub-microlitre DNA analysis
    Designed for use with several Thermo UV/vis spectrophotometers, including the Spectronic BioMate 3, the Spectronic Genesys 10 Bio, and the Evolution 300, the nanoCell accessory offers sub-microlitre analysis capabilities for DNA and protein analysis in the life science laboratory. The nanoCell allows users to obtain the maximum amount of information from minute sample volumes. This is especially critical in laboratories performing assays for nucleic acids, RNA/DNA and protein concentrations that wish to limit the amount of sample used for analysis.
News (8)
  • DNA testing for pet dogs
    DNA testing to establish the breed of pet dogs is now commercially available in Australia.
  • Identifying counterfeit products by their DNA
    DNA-embedded ink used in packaging may become the solution that authorities and companies need to combat counterfeit products.
  • Rapid DNA testing
    University of Rochester Chemistry Department researchers have developed a method for rapidly testing DNA. The method can be used to help forensics labs identify criminals, test ponds and pools before children swim in them, and identify harmful genetic sequences in medical research, to name only a few applications. The procedure quickly and inexpensively identifies genetic sequences in any sample of DNA.
  • Application to characterise structure of DNA molecules
    An application to enable more precise measurement of the location of a fluorescent label in a DNA layer has been developed by a team of researchers from Boston University in the US.
  • Presidential DNA sheds light on ancestry
    Research into the ancestry of US President, Thomas Jefferson has thrown new light onto his ancestry and the migration of a rare class of DNA.
  • Decoy makes drug effective against super bug
    A DNA-based therapy could speed the development of effective drugs to combat antibiotic-resistant super bugs.
  • DNA-damage test could aid drug development
    Researchers from Massachusetts Institute of Technology and Whitehead Institute have developed a cell culture test for assessing a compound’s genetic toxicity that may prove dramatically cheaper than existing animal tests. This assay would allow genetic toxicity to be examined far earlier in the drug development process.
  • RNAi services
    Invitrogen and genOway have signed a co-marketing agreement around RNAi related services. Under the terms of the agreement, Invitrogen and genOway will combine Invitrogen's expertise in RNAi research technologies with genOway's portfolio of transgenesis technologies and RNAi in vivo experience to create a services platform offering RNAi vector design through the creation of RNAi transgenic rodent models.
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