Cytek Biosciences Ghost Dye viability dyes

Monday, 08 July, 2024 | Supplied by: Sapphire Bioscience

When dead cells are included in flow cytometry analysis, it can result in high background and non-specific staining leading to false positives. To ensure accurate data, it is crucial to exclude dead cells from analysis. Viability dyes allow dead cells to easily be removed from analysis to improve data quality.

With Ghost Dye viability dyes, from Cytek Biosciences’ Tonbo Reagents portfolio, only live cells are analysed in experiments. The dyes come in a ready-to-use format, are easy to incorporate into staining protocols and are resistant to subsequent washing, fixation and permeabilisation.

The dyes can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. They irreversibly bind free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react significantly more with the viability dyes than viable cells in the same sample and therefore will exhibit greater fluorescence intensity, allowing exclusion of these cells from analysis.

Phone: 02 9698 2022
Related Products

Enzo Life Sciences SCREEN-WELL Autophagy library

Enzo Life Sciences' SCREEN-WELL Autophagy library contains 94 compounds with defined...

Bio-Rad Laboratories anti-biotherapeutic and anti-MMAE antibodies

The company's range of ready-made anti-biotherapeutic antibodies enables the development of...

List Labs Clostridium difficile toxins

List Labs manufactures highly purified native Clostridium difficile toxins — toxin...

  • All content Copyright © 2024 Westwick-Farrow Pty Ltd