Molecular Devices ScanLater Western Blot Detection System

Friday, 06 March, 2015 | Supplied by: Bio-Strategy Pty Ltd

The ScanLater Western Blot Detection System enables western blot membrane detection in a multimode microplate reader platform. The system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Assay Kit and image acquisition powered by SoftMax Pro Software. Users can install the ScanLater Western Blot Detection Cartridge in either the SpectraMax i3 or SpectraMax Paradigm systems in minutes, adding western blot detection capability, rather than investing in dedicated western blot detection systems.

The ScanLater Assay is a time-resolved fluorescence (TRF)-based western blot detection assay. TRF is an optimal detection method, as reduction in stray excitation light results in low background and high sensitivity. ScanLater Western Blot Kits contain Europium-labelled secondary antibodies designed to work with existing primary antibodies without further optimisation. This substrate-free method of western blot detection not only outperforms traditional chemiluminescence and fluorescence-based western blot detection, it significantly extends signal stability, allowing membrane scanning at any time.

The kit contains all components needed to go from transfer to detection. There are three kits available: anti-rabbit, anti-mouse or Streptavidin. Each kit contains enough Europium-labelled secondary antibody, blocking buffer and wash buffer to run 30 mini-sized membranes. All kits are validated for use on the SpectraMax i3 or SpectraMax Paradigm microplate readers.

Phone: 1800 00 84 53
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