NanoString GeoMx human protein assays

Tuesday, 06 April, 2021 | Supplied by: Bio-Strategy Pty Ltd



NanoString has expanded high-plex protein assay support from tens to now hundreds of validated proteins to be analysed from a single tissue section with spatial resolution on the GeoMx Digital Spatial Profiler (DSP) instrument. This breakthrough is made possible by harnessing Illumina next-generation sequencing (NGS) readout, enabling multi-analyte (RNA and protein) analysis for both nCounter and NGS readout.

Users select any particular cells of interest using the GeoMx microscope and quantify the protein or RNA molecules those cells express with digital precision. The NGS readout protein assays have been tested for multi-plex performance on both formalin-fixed paraffin embedded (FFPE) and fresh frozen (FF) tissue. Proteins are targeted using antibody conjugation and assays are configurable by ordering pre-optimised antibody sets in mix-and-match modules of ~10 functionally related proteins each.

Currently available content covers applications in immuno-oncology and future content releases will cover immunology and neuroscience. Custom-barcoded antibodies from NanoString or Abcam can also be spiked into the high-plex assays to further increase the targeting potential of the system.

Online: www.bio-strategy.com
Phone: 1800 00 84 53
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