NanoString Technologies nCounter Analysis System

Thursday, 25 July, 2013 | Supplied by: Bio-Strategy Pty Ltd



The NanoString nCounter Analysis System uses fluorescent barcodes to detect and count single nucleic acid molecules by direct hybridisation and can be multiplexed for up to 800 different targets in a sample. This high level of multiplexing and pure digital detection avoids the pitfalls of enzymatic steps such as reverse transcription and PCR while offering high precision and reproducibility. In addition, NanoString technology is amenable to challenging sample types such as formalin-fixed, paraffin-embedded (FFPE) tissue, cell lysates and raw biofluids.

An expanding list of applications includes gene expression, single-cell gene expression, miRNA expression and fusion gene analysis. DNA applications include copy number variation (CNV), ChIP and haplotyping.

The technology is a suitable choice for validation studies and translational research applications. It offers the three key characteristics required to accelerate genomic discoveries into robust clinical assays: mid- to high-multiplexing capacity capturing the key findings of NGS discovery; digital readout for direct comparison with NGS; and an automated, enzyme-free workflow for fast and accurate quantitation of thousands of samples in retrospective-prospective studies using FFPE samples.

Key benefits include: no amplification (except for single-cell assays), RT or sample partitioning; digital counting of single molecules for low noise data; reproducible with wide dynamic range and high precision; expression calls in 10 min (no compute clusters); simple workflow - up to 800 targets in a single tube.

Online: www.bio-strategy.com
Phone: 1800 00 84 53
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