Rescan Confocal Microscope

Thursday, 01 June, 2017 | Supplied by: SciTech Pty Ltd


Re-scan confocal microscopy (RCM) is a super-resolution technique based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD camera. The RCM is an economical alternative to a confocal microscope and allows users to upgrade new and existing microscopes.

The Rescan Confocal Microscope from SANE Asia includes two scanning-mirror sets: the scanner and the re-scanner. While the scanner scans the specimen, the re-scanner writes the emission signal on the camera chip.

RCM has an axial resolution of 600 nm and lateral resolution of 170 nm at 488 nm excitation, as well as a quantum efficiency of 80–95% (depending on the camera). It also has a scan speed of 1 fps at 512 x 512 resolution images and is flexible in its configuration, with excitation wavelengths using up to four lasers (eg, 405, 488, 561 and 632 nm) and emission wavelengths with up to four emission bands.

The technology is useful for biological applications where the combination of high resolution and high sensitivity is required.

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