Malvern Panalytical Creoptix WAVEsystem for label-free binding kinetics and affinity analysis

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A common challenge in the field of biological drug discovery and development is understanding the behaviour of biologics such as antibodies in serum or plasma to determine their stability and performance. Conventional bioassays, like surface plasmon resonance (SPR) or biolayer interferometry, have relied on enzyme or fluorescent labels to detect and monitor biomolecular interactions, but these methods tend to capture just one point in time and are fraught with non-specific binding issues that can skew results.

Malvern Panalytical’s Creoptix WAVEsystem is a label-free technology that is designed to deliver deep insight into previously undetectable interactions. Kinetic rate parameters and affinity constants (k_a, k_d, K_D) along with binding specificity are derived in real time from even the most challenging sample types in a wide range of biological matrices with a resolution that’s not possible with other methods, the company says.

The power behind the Creoptix WAVEsystem is in the patented Grating-Coupled Interferometry (GCI) technology and non-clog WAVEchip microfluidics system. GCI measures the evanescent wave across the entire sensor surface and is not affected by temperature drifts or vibrations, allowing for sensitive measurement. Large molecules up to 1000 nm with high affinity and slow dissociation can be measured simply (even native proteins in complex matrices) without purification. The chip design also allows for determination of off-rates up to 10 s^-1, meaning the kinetics of weakly binding fragments can be studied. The waveRAPID (Repeated Analyte Pulses of Increasing Duration) method can also be used to increase throughput compared to multi-cycle kinetics, particularly in screening applications, by generating a pulsating concentration profile.

The addition of two new wizards to the WAVEcontrol software aids to simplify and streamline workflows. They include ligand screening — a fast and flexible way to screen and characterise antibodies; and calibration-free concentration analysis (CFCA) — a quick and easy calibration-free approach to quantify active protein concentration.

For more information: https://www.atascientific.com.au/products/creoptix-wave/.