Biomedica NT-proBNP ELISA for determination of heart failure

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NT-proBNP brain natriuretic peptide, a member of the cardiac natriuretic hormones (CNHs) family, is secreted by the hemodynamically stressed heart, mainly in response to myocardial stretch induced by volume overload. The NT-proBNP ELISA from Biomedica provides reproducible results with an easy-to-use, convenient 96-well protocol (5 standards, 1 control). The kit has been designed for manual use or can be adapted to open ELISA systems.

BNP is synthesised as a 108 amino acid residue precursor and, upon release into circulation, is cleaved into an N-terminal fragment (NT-proBNP) and the biologically active hormone (BNP-32). NT-proBNP has a longer half-life in circulation (120 min) compared with the C-terminal BNP peptide (20 min). Therefore, the measurement of NT-proBNP offers good stability in serum/plasma, giving reliable results.

NT-proBNP has clinical significance for: detection of left ventricular dysfunction (LVD); differentiation between chronic obstructive pulmonary disease (COPD) and chronic heart failure (CHF); risk stratification after myocardial infarction (AMI); therapy monitoring of heart failure patients. The ELISA is CE marked, allowing for possible IVD use, and has a wide dynamic range which enables accurate detection of early stages of heart failure.