Enzo Life Sciences cortisol ELISA kit

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The cortisol ELISA kit from Enzo Life Sciences can be used with a large variety of sample types and produces highly reproducible results for stress, autoimmune disease and cancer research.

The ready-to-use, liquid, colour-coded reagents provided are safe and non-radioactive and reduce error in the lab. The broad dynamic range lets users accurately measure cortisol levels in a variety of sample matrices including saliva, culture supernatants, plasma, serum and urine from any species.

The product is a competitive immunoassay for the quantitative determination of cortisol in biological fluids with results in 3 h. The kit uses a monoclonal antibody to cortisol to bind, in a competitive manner, cortisol in a sample or an alkaline phosphatase molecule which has cortisol covalently attached to it.

After incubation at room temperature, the excess reagents are washed away and substrate is added. The enzyme reaction is stopped and the yellow colour generated is read on a microplate reader at 405 nm. The intensity of the bound yellow colour is inversely proportional to the concentration of cortisol in either standards or samples. The measured optical density is used to calculate the concentration of cortisol.