Readily3D Tomolite 3D volumetric bioprinter

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The Tomolite is a 3D bioprinter produced by Readily3D, a manufacturer based in Switzerland, for rapid, contactless, high-viability, tomographic bioprinting. It uses volumetric bioprinting technology to produce bio-ink/organic parts using liquid feedstock. Tomolite gently shapes sensitive cells and biomaterials into complex structures at high speed and without impairing their viability. Creating and studying biological systems becomes easy, with applications in bioprinting, biofabrication, nanomedicine and tissue engineering.

Volumetric tomographic 3D bioprinting rapidly solidifies photosensitive inks in three dimensions, using shaped light beams from multiple angles. As the entire build volume is illuminated simultaneously, centimetre-scale biological systems are produced in just tens of seconds with a high throughput of >10 cm³/min. After printing, the object is simply separated from the uncured ink and collected. The printing method is light-based, so it does not induce any shear stress on the printed cells. The low photoinitiator content (eg, 1 mg/mL LAP) and low light dose (<600 mJ/cm²) make tomographic bioprinting a cell-friendly technique.

Volumetric printing not only preserves cells but also makes research efficient by simplifying design iterations and statistical studies. An indicative print time for the Tomolite is 20–120 s (depending on the material) and hydrogels can be shaped in as little as 30 s with 28 µm pixel size resolution.

Apparite software directly imports STL files of the construct and gives full control over object and print parameters. Design is not limited to simple grids. Whether thick or thin, hollow or overhanging, complex organic shapes are printed easily with tuneable porosity and vasculatures. The printer is cell- and organoid-friendly, maintaining high viability (>90%) with low light dose. Light intensity of 1 to 20 mW/cm² (average at container) is possible. Two models are available — the standard model and the performance model — with wavelengths of 405 nm ±5 nm (standard model) or 400 nm ±1 nm (performance model); other wavelengths can also be requested.

Printing is contamination-free as it is achieved through sealed, autoclavable glass vials. Compatible materials include hydrogels, acrylics and silicones. The Tomolite printer measures 27 x 30 x 67 cm.