Products
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The MycoTool PCR Mycoplasma Detection Kit from Roche can be used to obtain results in hours by using PCR instead of culture-based testing.
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Lonza has available primary, diseased primary and stem cells. Research shows that non-transformed, non-immortalised cells isolated directly from tissue provide conditions that closely simulate a living model and yield more physiologically significant r...
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Lonza, with extensive experience in protein and nucleic acid electrophoresis, has an extensive range of products optimised for the unique requirements of most critical molecular biology techniques.
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Clonetics NHAC-kn-Normal Human Articular Chondrocytes are performance tested and positive for type ll collagen and sulfated proteoglycans after differentiation.
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Lonza’s Resource Notes - Fall 2011 are now available for download from its website: http://www.lonza.com/go/literature/3704.
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The ProSieve EX running and transfer buffer system is said to speed up separation and transfer time by over 90 min using standard protocols.
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Lonza has available Nucleofector kits and protocols for adherent cells, including neurons. The company’s optimal protocols provide good adherent Nucleofection of primary neurons using Lonza Nucleofector systems.
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The CalciFluor Bone Resorption Assay kit provides reagents for the sensitive and quantitative measurement of in vitro bone resorption in a rapid and homogeneous format.
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Electroporation-based methods have so far required cells to be in suspension for transfection. The Nucleofector technology in Lonza’s 4D-Nucleofector X unit and Y unit allows direct Nucleofection of adherent cells, eg, neurons.
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The Lonza Spring 2011 resource notes are available for download from www.lonzabio.com/.
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The AdipoRed adipogenesis assay reagent can be used for assessing the effect of compounds on the differentiation of preadipocytes or on lipid utilisation in mature adipocytes.
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TheraPEAK FGM-CD fibroblast growth medium - chemically defined and without phenol red and antibiotics - is designed to support the growth and proliferation of primary human dermal fibroblasts in culture.
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Clonetics Diabetic Type I and Type II human skeletal muscle myoblasts (HSMM) are isolated from donors diagnosed with either Diabetes Type I or Diabetes Type II. Additional donor information is available by contacting Lonza’s Scientific Support.
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PAGEr Gold Precast Gels are designed for optimal separation of a wide range of proteins. They contain a stacking gel and a resolving gel for separating denatured and native proteins.
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Primary human bone marrow hematopoietic progenitors are multipotent cells that can differentiate into several lineages. EasyDiff supplements allow differentiation of CD34+ cells into three different hematopoietic lineages (myeloid, erythroid and megaka...
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The AdipoLyze Lipolysis Detection Assay is a fast, sensitive, fluorescent in vitro assay that can be used for detecting small quantities of glycerol in cells undergoing lipolysis.
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GelStar nucleic acid gel stain is a highly sensitive fluorescent stain for detecting both DNA and RNA. It detects as little as 20 pg of dsDNA or 3 ng of RNA.
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The Platinum UltraPak Bioprocess single-use containers can be used for packaging of cell culture media, reagents and buffers as well as containment and storage vessels for final product yielded from downstream manufacturing processes.
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Using cutting-edge Protein-fragment Complementation Assay technology from Odyssey Thera, Lonza is able to offer profiling services that can shape and influence critical decisions about drug candidates.
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Various Nucleofector Kits and corresponding Amaxa optimised protocols are available for the transfection of human melanocytes using the different Nucleofection platforms.
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NHEM-Neo (Neonatal) and NHEM-Ad (Adult) normal human epidermal melanocytes with optimised growth media can be used in a variety of skin-related research including: pigmentation and cosmetics testing; cellular differentiation; antigen expression; melano...
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Lonza’s bone marrow and cord blood mononuclear cells are prepared by centrifugation in a density cell separation medium (Ficoll-Paque, Amersham Pharmacia).
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The GeneSieve Bioanalysis software package enables users to find the most appropriate StellARray qPCR arrays or gene lists associated with a particular research area or gene
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MetaPhor Agarose is an intermediate melting temperature agarose that is claimed to provide twice the resolution capabilities of the finest sieving agarose products.
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The Lucetta single-sample luminometer can be used for the detection of bioluminescence and chemiluminescence.
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The BioWhittaker ProNS0 is a chemically defined, protein-free medium, with HEPES and Pluronic and without L-glutamine, phenol red or cholesterol.
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GelBond film is a transparent, flexible polyester film designed to support agarose gels.
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Poietics osteoclast precursors yield ≥70% conversion to mature and functional osteoclasts when differentiated using Poietics media.
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The Human Diabesity 384 StellARray qPCR Array can be used to profile the expression pattern of genes that have been identified as important in diabetes and obesity research. Users can perform an extensive gene expression profile of Type 2 diabetes and ...
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The Nucleofector kits and corresponding Amaxa optimised protocols are now available for the transfection of human keratinocytes.
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The PPiLight assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate (PPi).
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Human blood is obtained from normal human donors who test negative for hepatitis B surface antigen (HbsAG), antibodies to hepatitis C (HCV) and human immunodeficiency virus I & II (HIV-I and HIV-II).
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The Clonetics KGM-Gold NHEK normal human epidermal keratinocytes have longer guaranteed life spans than Lonza’s original KGM or KGM-2 cells.
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PC-1 is a low-protein, serum-free medium that can be used for the culture of primary cells and anchorage-dependent cell lines.
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Clonetics human cardiac fibroblasts from atrial and ventricular tissue are isolated from adult heart tissue and cryopreserved at passage 2 with ≥500,000 cells per vial.
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Various Nucleofector kits and corresponding Amaxa optimised protocols are available for the transfection of human T cells using the different Nucleofection platforms.
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The 384 StellARray qPCR array is designed to profile the expression pattern of genes that may increase susceptibility, contribute towards the progression or provide a neuroprotective role in Alzheimer’s disease.
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The PDELight HTS cAMP phosphodiesterase assay kit, a generic, homogeneous assay, can be used in high-throughput screening of phosphodiesterase activity.
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I.D.NA agarose is a high gel strength, low electroendosmosis agarose developed for consistent resolution of DNA in DNA identity testing.
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Clonetics human intestinal myofibroblasts, cryopreserved at passage 2 with ≥500,000 cells per vial, are available. The recommended media kit is SMGM-2 smooth muscle growth media.
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Osteoclasts are large, multinucleated cells that play an active role in bone resorption. The Poietics human osteoclast precursor cell system is designed for use in high-throughput research applications in osteoporosis, bone resorption and other bone-re...
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ApoGlow offers sensitive, safe and rapid 20 min determination of apoptosis, necrosis and cell proliferation in a single assay. The convenient 96-well format can be fully automated. The kit is suitable for both adherent and non-adherent cells.
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Poietics normal human neural progenitor cells and media can be useful for research applications in drug development, neurotoxicity, neurogenesis, electrophysiology, CNS function and neurotransmitter disorders.
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Clonetics NHA-normal human astrocytes are useful for experimental applications in neurogenesis research, cell physiology, injury, astrocyte-mediated neurotoxicity, drug development, Parkinson’s and Alzheimer’s diseases.
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ProFreeze serum-free freezing medium has been specifically formulated for cryopreserving cells that have been propagated in serum-free media.
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The 2011-2012 Lonza BioResearch catalogue 2011-2012 can be ordered online at https://www.lonzabio.com/?id=594.
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The 4D-Nucleofector system video shows how easy it is to work with the system.
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Lonza resource notes are now available for download at http://www.lonzabio.com/.
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The PyroGene recombinant Factor C assay (rFC) assay represents a reliable, sustainable alternative to LAL.
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Poietics rat mesenchymal stem cells (rMSC) are isolated from bone marrow taken from the femur and tibia of female Fischer 344 rats.
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ProSieve colour protein markers contain sets of proteins and dyes for use as visible markers in SDS-PAGE gels to help monitor the efficiency of separation. In Western blotting they confirm transfer has occurred from the gel to the membrane.
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The Clonetics prostate smooth muscle cell system includes normal human prostate smooth muscle cells (PrSMC) and optimised growth medium.
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BioWhittaker UltraDoma with L-glutamine is a protein-free formulation designed for the cultivation of murine, human and chimeric hybridomas, myeloma growth and monoclonal antibody production.
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Insect-XPRESS medium is a protein-free formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
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Poietics ECFCs human endothelial colony-forming cells are a unique population of highly proliferative cells isolated from human umbilical cord blood that demonstrate endothelial behaviour in culture.
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Lonza has available a broad selection of cryopreserved primary human diseased airway cells including, bronchial epithelial, smooth muscle cells and lung fibroblasts from both COPD and asthmatic donors.
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Clonetics HSeC-Human Sertoli Cells are an asset to research involving male infertility and reproductive toxicology, xeno/allograft cotransplantation, immune suppression and many other applications.
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Lonza’s Australian-origin foetal bovine serum can be used in cell culture media, production of biological/diagnostic products and other laboratory procedures.
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The OsteoImage mineralisation assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralisation. The assay is based on specific binding of the fluorescent OsteoImage staining reagent to the hydroxyapatite portion of bone-like nodules ...
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Clonetics Mouse Hippocampal Neurons are a useful tool in the study of many neural diseases and disorders.
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Clonetics rat hypothalamus neurons can be used in the study of neuroendocrine-related diseases and disorders.
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The retinal pigment epithelium (RPE) comprises hexagonal cells organised into a monolayer that is densely packed with pigment granules. RPE cells play a critical role in visual function and photoreceptor viability.
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The ViaLight Plus cell proliferation and cytotoxicity bioassay assay kit uses bioluminescent detection of cellular ATP as a measure of viability, claimed to be the most accurate, effective and direct way of determining the number of living cells in cul...
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Lonza produces a variety of sera products for use in cell culture media, production of biological and diagnostic products, and other laboratory procedures that require quality serum.
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The TheraPeak MSCGM-CD mesenchymal stem cell growth medium is chemically defined and serum free, and contains L-glutamine, no phenol red and no antibiotics.
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The X-VIVO 10 chemically defined, serum-free hematopoietic cell medium was designed to support the generation of lymphokine activated killer (LAK) cells in a serum-free environment.
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The ToxiLight bioassay kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of adenylate kinase (AK) from damaged cells.
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The Amaxa Nucleofector technology is claimed to be the first efficient non-viral transfection method for primary cells and hard-to-transfect cell lines.
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Embryonic stem cell-derived cardiomyocytes have many of the phenotypic properties of primary cardiomyocytes and show great potential to model human diseases. The Cor.At cells are cardiomyocytes derived from transgenic mouse embryonic stem cells and are...
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Mesenchymal stem cells (MSCs) are pluripotent cells that can differentiate into several cell types and play a vital role in several research applications eg, cell differentiation and gene regulation, gene therapy and transplantation studies, and cell-b...
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Clonetics h NHEPS human hepatocytes are available as cryopreserved ampoules containing 3-6 million cells.
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The MycoAlert mycoplasma detection kit includes reagents and an instruction manual for the detection of mycoplasma contaminations in cell cultures.
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The QCL-1000 endpoint chromogenic LAL assay is a claimed fast and quantitative endpoint assay for the detection of gram-negative bacterial endotoxin in a wide variety of samples.
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The FlashGel System for RNA is optimised for the unique requirements of RNA and is a claimed fast and sensitive tool for analysis of sample integrity. It is suitable for verification and analysis of total RNA, quick checks of native RNA and checking RN...
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A claimed robust and versatile serum-free medium formulation for high- and low-density normal human epidermal keratinocyte (NHEK) cultures, KGM-Gold keratinocyte growth medium is designed to support both clonal growth and high-density keratinocyte prol...
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Lonza supplies a variety of primary cells isolated from normal peripheral blood, cord blood and bone marrow along with media specifically developed for culturing primary immune cells.
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The Clonetics HUVEC-XL assay-ready human umbilical vein endothelial cells are primary HUVECs packaged at 10 million cells per vial that have been expanded to passage-3 and retain their endothelial properties as indicated by rigorous endothelial-specifi...
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The MycoZap Plus-CL, Plus-PR and Prophylactic cell culture antibiotics have been released.
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The Poietics human adipose-derived stem cell system provides 1 million viable cells cryopreserved in an ampoule with ≥95% purity.
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Lonza agaroses are claimed to provide quality, purity and performance for electrophoresis.
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Amaxa’s Nucleofector technology is a highly efficient non-viral method for transfection, based on two components: the Nucleofector device that delivers unique electrical parameters and Nucleofector kits that contain cell-type-specific Nucleofecto...
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The StellARray Gene Expression System’s qPCR system helps users to focus on the genes relevant for their individual research area while freeing gene expression profiling from unwanted bias.
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The B-ALI bronchial air liquid interface medium BulletKit provides scientists with a tool to investigate new areas of bronchial epithelial research in a more physiologically relevant way.
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The B-ALI bronchial air liquid interface medium BulletKit provides scientists with a tool to investigate new areas of bronchial epithelial research in a more physiologically relevant way.
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Lonza has completed the acquisition of the preclinical cell and molecular biology assets of Simbiosys Biowares India, a biology outsourcing company, expanding its cell-biology platform.
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Potelligent CHOK1SV, a host cell line for manufacturing recombinant antibodies, combines BioWa’s glycosylation Potelligent technology with Lonza’s GS Gene Expression System and CHOK1SV.
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The Lonza StellARray Gene Expression System can be used to reveal gene expression level changes with unbiased significance.
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The StellARray Gene Expression System consists of three products: the GeneSieve Query; the StellARray qPCR 96/384-well Arrays; and the Global Pattern Recognition (GPR) analysis tool.
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Articles
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Clonetics Prostate Smooth Muscle Cell Systems contain Normal Human Prostate Smooth Muscle Cells (PrSMC) and optimized medium for their growth. Each System can generate PrSMC cultures for experimental applications in benign prostatic hyperplasia (BPH), prostitis and prostate cancer.
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The Clonetics B-ALI Bronchial Air Liquid Interface Medium BulletKit provides a model system to investigate bronchial epithelial cells in a more physiologically relevant way.
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Direct DNA recovery using the FlashGel Recovery System eliminates agarose gel preparation, band excision, and purification, and delivers highly efficient recovery, free from inhibitors and UV-induced damage, in a simple 5-10 minute protocol.
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Instructions for use are provided in this article for protocols for converting cells to serum-free culture.
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Cryopreservation may compromise cell quality and performance. Performance of the cells CANNOT be guaranteed after cryopreservation.
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Bone is a rigid, yet dynamic organ that is continuously molded, shaped, and repaired. Old bone is degraded by osteoclasts and replaced with new bone by osteoblasts, a process called remodeling or bone turnover.
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Reprogramming of somatic cells to pluripotent iPS (induced pluripotent stem) cells promises to revolutionize regenerative medicine.
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In this paper, we present three application examples demonstrating the utility of the StellARray Gene Expression System to reveal gene expression level changes in diverse biological contexts such as toxicology, cancer, and stem cell differentiation.
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Transfection methods are widely used to study miscellaneous aspects of cell biology.
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Lonza’s R&D efforts and collaboration with many clinical trials have provided Lonza with the tools and expertise to support the developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies.
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To date, in vitro preclinical electrophysiological studies rely mainly on recombinant cell systems like injected oocytes or mammalian cell lines expressing only one specific ion channel.
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Nucleofection has become a method of choice whenever transfection of primary cells or difficult-to-transfect cell lines is required.
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Cultured human keratinocytes provide a model to study a number of medically relevant topics such as metabolism, nutrition, toxicity, differentiation, various epidermal diseases, and the treatment of serious burns.
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DNA transfer into human embryonic stem cells (hESC), derived from the inner cell mass of a four day old embryo and able to differentiate into derivatives of all 3 germ layers, is limited by their general resistance to common methods used to introduce exogenous DNA.
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Assessment of RNA quality/integrity is key to consistent performance in a variety of uses, including microarrays, RT-PCR, and RTqPCR.
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The importance of recombinant protein expression and production for structural and functional protein studies is constantly growing.
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Dissociation and production of rat primary dorsal root ganglion (DRG) cells for culture is labour intensive and offers low yield.
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Label-free, cell-based assay platforms are innovative tools for assessing the integrated functional responses of cells to drug-like compounds.
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Primary cells or cell lines derived from solid tissue, eg endothelial, epithelial or neuronal tissue, typically grow in adherence when transferred into cell culture.
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