HDACs are typically found in multiprotein complexes and are tightly regulated by subcellular localisation, phosphorylation, and likely by other mechanisms.
The HDAC Fluorimetric Cellular Activity Assay from BIOMOL International allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation.
It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity. The Fluor de Lys HDAC Substrate is cell permeable and thus is suitable for high-throughput cell-based deacetylase assays.
This kit provides the reagents and protocols needed for determining rates of intracellular deacetylase activity with cultured cells. The kit includes Fluor de Lys Substrate, Fluor de Lys Developer, Trichostatin A (HDAC inhibitor), Nicotinamide (Sirtuin inhibitor), Fluor de Lys Deacetylated Standard, HDAC Assay Buffer, Cell Lysis Buffer, microplates, and a detailed protocol.
