Bio-Rad ChemiDoc MP Imaging System and Starbright fluorescent secondary antibodies

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The ChemiDoc MP Imaging System is designed for multiplex fluorescent western blotting. With triplex acquisition, the product can detect up to three proteins simultaneously to gain more data per sample and minimise stripping and reprobing.

The ChemiDoc MP has five fluorescent channels spanning the entire red, green, blue (RGB) and near-infrared (NIR) spectra, compatible with a wide range of supported fluorophores to simplify experimental design. This includes Bio-Rad’s Starbright Secondary Antibodies, highly cross-adsorbed IgGs labelled with an ultrasensitive near-infrared fluorophore, suitable for low abundant proteins.

The system also fully supports Bio-Rad’s Stain-Free technology, allowing rapid fluorescent detection of protein on gels and blots and, therefore, multiple points at which to visualise and validate results. This permits loading control normalisation utilising total protein concentration and eliminates the need for housekeeping proteins.

The ChemiDoc MP also enables sensitive chemiluminescent detection with ECL substrates and gel documentation applications.