Bruker Luxendo LCS SPIM light-sheet microscope for large cleared samples

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The Bruker Luxendo LCS SPIM is a compact, benchtop light-sheet microscope using selective plane illumination microscopy (SPIM) and designed for fast and gentle 3D imaging of large cleared samples (LCS). The product is optimised for 3D imaging of large tissue structures, crucial when studying nervous system networks, when analysing organ development or when investigating tumour structure and tumorigenesis in oncology.

Typical applications of the microscope include imaging of large cleared-tissue samples, eg, whole mouse brains to visualise complete neuronal and vascular networks, and organs. It combines two-sided illumination and single detection with a novel optical concept for high-throughput imaging of large cleared samples and resolution down to 1 µm in 3D.

The modular LCS SPIM has been designed to be compatible with a broad variety of clearing solutions and sample sizes. The novel sample mounting approach and innovative optical design enable ultrafast acquisition times and minimise sample distortions while seamlessly integrating into existing clearing and sample preparation pipelines.

The product features a removable standard quartz crystal cuvette for gentle sample mounting, easy access and compatibility to all types of clearing solutions. A motorised sample stage and a programmable optics concept for fast 3D scanning of the light-sheet through the sample minimises the mechanical stress imposed on the sample. A cuvette filled with the clearing solution holds the specimen and is positioned on the sample stage.

The typical dimensions of the cuvette (20 x 20 x 20 mm) can be selected to accommodate specimens of different sizes, enabling sample imaging up to 40 mm long. This design minimises motion-induced sample distortions, while its novel optical configuration enables high acquisition speed.