Jasco J-1500 circular dichroism (CD) spectrophotometer

Friday, 01 December, 2017 | Supplied by: ATA Scientific Pty Ltd

The Jasco J-1500 CD spectrophotometer allows researchers to carry out measurements with a high signal-to-noise (S/N) ratio, especially for highly absorbing materials found in the far-UV, by incorporating several of the latest advances.

CD spectroscopy is an essential tool for the structural analysis of biological samples from small organic molecules and nucleic acids to higher molecular weight proteins, polymers and polypeptides. It provides an indication of the percentage of the molecule that is in each conformation (α-helix, β-sheet, β-turn, etc), which can then be used with other analytical methods to determine the complete structure. Denaturation and other conformational changes, along with the thermodynamics of such changes, may also be studied within the spectral range.

The limit of detection of a circular dichroism (CD) spectrophotometer (or any spectrophotometer) is determined by its S/N characteristics: the better the S/N, the better its limit of detection. The Jasco J-1500 CD permits the measurement of a CD spectrum in the vacuum-UV region down to 163 nm. The enhanced light throughput and digital lock-in technology allows for a small signal in a noisy environment to be detected.

Additionally, the optimisation of the nitrogen purge efficiency helps to reduce the amount of oxygen in the optical bench so that it does not bury the sample signal. The quality of spectral data obtained, including data obtained at shorter wavelengths, is said to improve the accuracy of protein secondary structure analysis.

For more information: https://www.atascientific.com.au/products/jasco-cd-j-1000-series/.

Online: www.atascientific.com.au
Phone: 02 9541 3500
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