Malvern OMNISEC and SEC MALS 20 for protein aggregate analysis

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The Malvern OMNISEC is a multidetector gel-permeation/size-exclusion chromatography (GPC/SEC) system. It is said to be a complete solution for the molecular characterisation of synthetic polymers, natural polymers and polysaccharides and proteins. It combines refractive index, UV/Vis absorbance, light scattering and viscometer detectors to measure concentration, molecular weight and intrinsic viscosity with high sensitivity.

Size-exclusion chromatography (SEC) has become a fixture in biopharmaceutical research and QC labs to provide molecular weight, size, shape, purity and additional information. Protein aggregates are of particular concern, as they can reduce product efficacy and stability and increase immunogenic risk. The combination of a multidetector SEC/GPC system, such as the OMNISEC, and the multi-angle light scattering SEC MALS 20, enables the measurement of the molecular weight of each aggregated species, improving product understanding and supporting biocomparability studies.

The sensitivity of the OMNISEC’s light scattering detector enables measurement of molecular weight as low as 200 Da, injection masses as low as 100 ng of material and samples with low dn/dc. The system is available as a detector-only option, which can be connected to any third-party GPC/SEC system. Alternatively, it forms part of a complete OMNISEC GPC/SEC system.