Eksigent cHiPLC-nanoflex chip-based platform and micro cHiPLC columns

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Eksigent’s cHiPLC-nanoflex is a chip-based platform that, in combination with the NanoLC-Ultra system, delivers good sensitivity, column-to-column reproducibility and ease of operation. The system is a ‘docking station’ for up to three microfluidic chips. Its flexible design and built-in 10-port nano valve allow for easy switching between different types of experiments such as direct injection and trap-loading.

Chips containing a nanoLC column, microLC column or trap column can be exchanged in seconds. A precise dead-volume-free connection is made every time as the cHiPLC column or trap column is automatically aligned with a special connector chip.

All alignment is achieved through the use of highly reproducible microfabrication techniques similar to those used in the microelectronics industry. Additionally, these techniques are used to define fluidic paths and to create a microfabricated weir structure for stationary phase particle retention. Chips are temperature controlled to guarantee reproducible retention times and improved separations.

The company’s micro cHiPLC columns decrease column run times and increase sample throughput with a larger diameter 200 μm ID format. The 200 μm ID cHiPLC columns allow samples to be run at higher flow rates and therefore with higher throughput. The larger diameter column also provides increased robustness, ease of use and sample loading capacity over traditional 75 μm ID nanoflow LC.

Benefits include: plug-and-play chip simplicity with the performance of a micro column; flexibility to switch between workflows and projects rapidly in multiuser labs; reproducible results from day to day, column to column and lab to lab.