Excelitas Technologies X-Cite FIRE illumination system for fluorescence microscopy

The X-Cite FIRE fluorescence illumination system by Excelitas Technologies is an arc lamp replacement for routine and advanced fluorescence imaging applications. It is claimed to have the broadest spectrum available in a white light LED for fluorescence microscopy and to rival traditional arc lamps for brightness, making it suitable for both compound and stereo microscopes.

In addition to its powerful output and broad DAPI to Cy7 spectral range, the product offers flexibility. Delivering light through a light guide alone or with a choice of more than a dozen microscope adaptors, the device can be installed on most new imaging systems or used to retrofit the microscopes labs have depended on for years.

Offering two models with a choice of UV wavelengths (365 or 385 nm), labs may choose the one that works with their preferred or existing DAPI filter sets. Each system includes intuitive fingertip control with speedDIAL, hands-free operation with a foot pedal, and USB and TTL inputs for automated applications.

The product’s high power in 500–600 nm for TRITC and mCherry enables fast imaging time and good excitation for dim specimens. Power levels for TRITC, Cy5 and Cy7 excitation are said to rival that of arc lamps and can handle everything from routine imaging to demanding high-speed applications.

Designed for use with liquid light guides, the system is compatible with modern light-guide-only microscope designs and can also be combined with Excelitas microscope adaptors to replace traditional lamp houses. It makes it possible to switch from mercury lamps to environmentally friendly LEDs without compromising on performance.