HAWK Biosystems Violet 3.0 all-in-one microscopy platform for QF-Pro technology

HAWK Biosystems’ Violet 3.0, an all-in-one microscopy platform for QF-Pro technology, has been designed to bring fluorescence lifetime imaging microscopy (FLIM) and Förster resonance energy transfer (FRET) to any laboratory. QF-Pro technology enables scientists to spatially map protein–protein interactions and protein post-translation modifications.

The comprehensive benchtop solution features a high-end modulated laser, a sensitive image sensor and a multichannel LED for immunofluorescence. Two stage inserts allow for the analysis of microwell plates or up to four standard microscope slides at a time. It is equipped with 10x, 20x and 40x dry objectives. It also has four LED channels — 365, 488, 585 and 635 nm — for the illumination of secondary biomarkers of interest.

One desktop licence of QF-Pro software is included, allowing users of all expertise to easily run the device and generate groundbreaking data. Users can load their samples and create maps of regions of interest (ROIs). The platform then sequentially acquires all mapped ROIs and calculates a QF-Pro score and image per region.

Post-acquisition analyses, such as the application of a threshold or change in lookup table (LUT), is automated, with the option of fine-tuning for more experienced users. All data can then be seamlessly exported into Microsoft Excel (data) or PowerPoint/PDF (images).