Hydrophilic interaction chromatography (HILIC) is suitable for separating and retaining very polar compounds that do not retain or have very limited retention on traditional reversed-phase packings. HILIC columns retain highly polar compounds with only small amounts of water in the mobile phase. These more volatile mobile phases increase sensitivity with LC/MS applications. Water is used as the strong solvent so increasing water content will elute peaks faster.
Alltech Alltima HP HILIC columns are optimised for HILIC separations, providing good retention and peak shape of highly polar compounds. Alltima HP HILIC is available in 1.5, 3 and 5 µm particle sizes as well as a variety of column formats, ranging from capillary to preparative.
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