Multiphoton imaging

Wednesday, 30 September, 2009 | Supplied by: Olympus Australia Pty Ltd


The Olympus FluoView FV1000MPE Multiphoton laser scanning microscope system offers bright clear imaging in living cells at depth within specimens.

At the core of the FV1000MPE technology is the optical design. A custom AOM has been incorporated to adjust laser light intensity, with rapid on/off switching. This is combined with an automatic beam expander that ensures optimal adjustment of the excitation laser beam diameter and maximal filling of the entry pupil of the microscope objective when changing excitation wavelengths and objectives.

Correcting for light refraction in the sample for deeper imaging is essential. Olympus has designed a multiphoton-dedicated 25X objective (the XLPLN25XWMP) with a correction collar that can be adjusted to compensate for any refractive index mismatch, allowing the formation of an focal spot deep within the sample without reducing energy density.

The widefield design into both the FV1000MPE non-descanned detectors and XLPLN25XWMP objective captures the maximum amount of fluorescence signal for highly efficient imaging. A transmitted fluorescence detector with a dedicated NA condenser allows for bright fluorescence imaging deep within a live specimen and also allows concurrent second harmonic generation imaging.

Online: www.olympusaustralia.com.au
Phone: 03 9265 5400
Related Products

CoolLED pE-300white LED illumination system for fluorescence microscopy

The system delivers powerful, broad-spectrum LED illumination designed for fluorescence...

BUCHI ProxiMate and ProxiScout NIR systems

ProxiMate is an at-line NIR instrument specifically designed for the food and feed industry,...

LICORbio Atlas for 2D and 3D cell imaging

Designed for both 2D and 3D assays, the product delivers high-content, high-throughput imaging in...


  • All content Copyright © 2025 Westwick-Farrow Pty Ltd