Phenomenex Yarra SEC-X300 1.8 µm UHPLC size-exclusion column

Phenomenex introduces the Yarra SEC-X300 — a high-resolution gel filtration/aqueous size-exclusion column for the separation of high molecular weight (HMW) biomolecules on HPLC and UHPLC systems. With a wide separation range of 10 K to 700 KDa and high efficiency, the product is suitable for separating and characterising monoclonal antibody (mAb) aggregates, antibody drug conjugates (ADCs) and biosimilars in drug discovery and development research.

The HMW focus of the device complements the low molecular weight range (1 K–450 KDa) of the existing SEC-X150 column, providing two versatile separation tools for biopharmaceutical research. The Yarra surface chemistry, combined with bio-inert column hardware, is said to reduce sample adsorption compared to other GFC/SEC columns currently on the market, providing improved recovery and more accurate quantitation of biomolecules. The SEC-X150 and -X300 media are both now available in 150 and 300 mm column lengths for analytical flexibility to increase speed or further increase resolution.

Because the 1.8 µm SEC-X150 and -X300 columns typically operate at backpressures below 3500 psi on traditional HPLC and UHPLC systems, they can deliver improved performance (greater resolution, better peak shape and faster analysis time) compared to existing 3 and 5 µm GFC/SEC column methods. They are designed and manufactured for stability, reproducibility and long product life.