Shimadzu LC-30A Nexera UHPLC liquid chromatography system

The LC-30A Nexera UHPLC gives high-speed and high-resolution analysis without sacrificing basic performance parameters such as precision, near-zero carryover, good linearity, stability and ruggedness.

The Nexera is suitable for use in various fields, covering high resolution and high-speed separation, as well as high-sensitivity detection.

The pumps offer a flow range from 0.0001 up to 3 mL/min with 130 MPa pressure and from 3.0001 up to 5 mL/min with 80 MPa pressure. This allows the use of longer columns for covering fast LC with high flow rates and short columns as well as highest resolution using long columns.

The microreactor-based mixer supports a small system volume and short gradient delays.

The autosampler provides reduced carryover compared to previous instruments by keeping the direct injection with isolated metering system principle using three rinse solvents, various rinsing modes and design changes at needle and needle seal. To increase the throughput, overlapping pre-treatment can be used. The pre-treatment capabilities expand the application range of this instrument.

For high-temperature LC analysis up to 150°C, it offers efficient pre-heating for solvents to enable stable and reliable conditions. The advanced intelligent heat balancer minimises band broadening during high-temperature analysis through the use of independently controlled heat zones within the column compartment, allowing accurate column temperature regardless of flow rate. The post-column cooler can reduce detector noise when equipped for high-temperature analysis.

In addition to UV and PDA detectors, it is claimed the most sensitive and fastest RF-20A/20AXS fluorescence detectors can be applied with Nexera.

The system can be used for semi-micro, conventional and UHPLC methods without changes on the flowline.