Thermo Fisher Scientific Hypersil Gold HILIC column range

Tuesday, 13 July, 2010 | Supplied by: Thermo Fisher Scientific

The hydrophilic interaction liquid chromatography (HILIC) technique allows complementary selectivity to reversed phase. With the ability to retain highly polar and hydrophilic compounds, the Hypersil Gold HILIC column range has been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns.

Available with 1.9, 3 and 5 µm particle sizes, the columns are suitable for applications within drug discovery, food testing and environmental analysis.

HILIC works by incorporating water in the highly organic mobile phase to form an adsorbed water-rich layer on the polar stationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions and hydrogen bonding mechanisms. Furthermore, the highly organic mobile phase contains low salt levels, making the columns suitable for use with electrospray mass spectroscopy.

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