Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer

The Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer features advancements that are designed to improve system sensitivity over previous generations and expand the product’s ability to characterise and quantify complex biomolecules and biological systems. These enhancements are suitable not only for native omics and translational research, but also for the structural and biopharmaceutical analysis of intact proteins and their complexes.

The real-time capabilities of the device use built-in, intelligence-driven data acquisition strategies to enhance experimental efficiency and ultimately accelerate tandem mass tagging (TMT) for multiplexed quantitative analysis of proteomes. Peptide spectra are searched in real time against a study-specific database. Those that match then proceed for further analysis, increasing the speed of analysis of results. In addition, the system extends structural analysis up to m/z 8000, which enables the isolation and selective dissociation of protein complexes into their individual components, as well as revealing further insights about their exact structure.

Users can benefit from: the ability to perform intact/top-down experiments and simplify spectra with proton transfer charge reduction to extract important structural insights; expanded protein coverage through advances in instrument design and use of the Thermo Scientific FAIMS Pro interface to enhance precursor ion selectivity; and unified hardware and instrument control software. Thermo Scientific SureQuant internal standard-triggered methods for ultrasensitive targeted protein assays provide the ability to rapidly develop and deploy targeted assays for large protein panels.

The mass spectrometer can be integrated into a laboratory’s routine workflows with the Thermo Scientific Almanac web-based application, which allows users to view instrument operation and data acquisition in real time and receive notifications on the status of runs. Almanac offers scientists the ability to conveniently improve visibility to the utilisation of their laboratory systems any time and from any location.