Thermo Scientific ProSwift C4 RP-5H column for LC-MS analysis

The Thermo Scientific ProSwift C4 RP-5H column, for liquid chromatography-mass spectrometry (LC-MS) analysis, uses a capillary design to offer labs with limited samples and complex mixtures a fast and high-resolution solution for the identification of protein samples, facilitating high-sensitivity proteomics and biotech applications. Offering a wide range of operational flow rates, alternative selectivity and low carryover, the product is suitable for rapid LC-MS analysis of protein samples.

The column incorporates a low-pressure monolith with a porous structure, enabling fast low-flow separations without sacrificing resolution. The monolith uses a butyl (C₄) methacrylate co-polymer that is less hydrophobic than PS-DVB columns, meaning there is less column fouling. This is important for MS users, where sensitivity is higher compared to UV and carryover can cause interference.

The porous co-polymer is said to give the flow channels higher permeability in the stationary phase than particulate columns, resulting in increased separation efficiency. Due to this structure, smaller ID columns of 50 μm can be used for analyses, increasing sensitivity.

The product is available in a range of column formats for nano, capillary and micro flow chromatography, offering good resolution for large, hydrophobic intact proteins across biopharma and research proteomics. This enables flow optimisation for increased MS sensitivity, as well as rapid separation of monoclonal antibody and complex protein samples.