Eurofins IDmyk comparative sequencing identification

Wednesday, 01 August, 2018 | Supplied by: Eurofins | ams


Genetic sequence identification

Microbial identification via genetic sequencing is useful for helping to provide identification for unknown isolates that are found to be present in critical areas in an aseptic setting. This is vital for both investigation purposes and regulatory requirement.

Genomic DNA is extracted directly from dead or alive bacterial colonies grown under any conditions. The 16S rRNA gene is amplified using universal primers and thermalcyclers. The amplified 16S rRNA gene product is sequenced using dye terminator cycle sequencing chemistry. The sequence reactions are analysed using automated DNA sequencers and software.

Unknown bacteria samples are identified using microbial identification software and compared against the Eurofins IDmyk Comparative Sequence Index database containing over 8470 entries. Routine bacterial identification is performed (by default) using the long sequence (1200–1400 base pairs) of the rDNA. The longer sequence allows for better discrimination of closely related species and therefore gives higher confidence in the results provided.

Data analysis can be done using either automated or manual modes. Outcome predictions are done using the phylogenetic tree tool. The system also has the ability to build user-defined and user-validated custom libraries.

With 8470 valid bacteria type strain entries, the Eurofins IDmyk Comparative Sequence Index database, which is proprietary to Eurofins, is said to be the largest database in the world. ​The Eurofins IDmyk fungal database (1650 species) complements the bacterial library.

For more information, click here.

Online: www.eurofins.com.au/biopharma-services
Phone: 02 9704 2300
Related Products

BSI enos Version 3 lab animal management software

The BSI enos Version 3 lab animal management software can be easily customised to match the...

Media Cybernetics Image-Pro v10 2D/3D/4D image analysis software

Image-Pro v10 analysis software makes it easy to capture, process, measure, analyse and share...

eDAQ EPU452 Quad Multi Function isoPod with USB

The eDAQ EPU452 Quad Multi Function isoPod with USB is a four-channel, user-configurable data...


  • All content Copyright © 2019 Westwick-Farrow Pty Ltd