Eurofins IDmyk comparative sequencing identification
Microbial identification via genetic sequencing is useful for helping to provide identification for unknown isolates that are found to be present in critical areas in an aseptic setting. This is vital for both investigation purposes and regulatory requirement.
Genomic DNA is extracted directly from dead or alive bacterial colonies grown under any conditions. The 16S rRNA gene is amplified using universal primers and thermalcyclers. The amplified 16S rRNA gene product is sequenced using dye terminator cycle sequencing chemistry. The sequence reactions are analysed using automated DNA sequencers and software.
Unknown bacteria samples are identified using microbial identification software and compared against the Eurofins IDmyk Comparative Sequence Index database containing over 8470 entries. Routine bacterial identification is performed (by default) using the long sequence (1200–1400 base pairs) of the rDNA. The longer sequence allows for better discrimination of closely related species and therefore gives higher confidence in the results provided.
Data analysis can be done using either automated or manual modes. Outcome predictions are done using the phylogenetic tree tool. The system also has the ability to build user-defined and user-validated custom libraries.
With 8470 valid bacteria type strain entries, the Eurofins IDmyk Comparative Sequence Index database, which is proprietary to Eurofins, is said to be the largest database in the world. The Eurofins IDmyk fungal database (1650 species) complements the bacterial library.
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