ChromoTek V5-Trap for the isolation of V5-tagged proteins

Friday, 01 October, 2021 | Supplied by: BioNovus Life Sciences

ChromoTek has introduced the V5-Trap, which is a Nanobody or VHH coupled to agarose, magnetic agarose and magnetic beads for immunoprecipitation (IP) and co-IP of V5-tagged proteins.

The V5-tag is derived from the P and V proteins of Simian virus 5 (SV5 — a paramyxovirus) and is a popular epitope tag for the capture and detection of V5-tagged proteins in yeast, bacteria, insect and mammalian cells. The V5-tag may be fused to either the N-terminus or C-terminus of a protein or be internal. The short linear peptide sequence of the V5-tag is GKPIPNPLLGLDST and has a size of about 1.4 kDa.

Benefit for IP include: very low background; no heavy and light antibody chains in gel; high specificity and high binding capacity; high stability even under harsh washing conditions; compatibility with N- and C-terminal and internal V5-tag; effective elution with V5-peptide; and the recombinant Nanobody enables consistent results.

The V5-Trap is a convenient, well-characterised and high-performing tool; it is ready to use for fast and specific IP of V5-tagged proteins. It is compatible with downstream applications including mass spectrometric (MS) analysis, ELISA and enzymatic assays. V5-Trap’s pulldowns provide pure extracts of V5-tagged protein without contaminating heavy and light chain peptides as can occur with the use of conventional antibodies. Effective elution of bound V5-tagged proteins can be conducted under denaturing conditions (for SDS-PAGE/WB, MS) or under gentle, native conditions using V5-peptide.

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