Proteintech CoraLite Plus-Tyramide reagents and detection kits
Proteintech’s CoraLite Plus-Tyramide dyes are fluorescent labelling reagents based on TSA technology. Upon interaction with HRP, the tyramides are oxidised into free radicals, which then allows the dyes to form covalent bonds with nearby tyrosine residues on the antigen of interest.
Compared to indirect staining methods, TSA dyes can enhance the fluorescent signal by several hundred-fold due to dye accumulation and direct labelling of the target. This makes them particularly useful for the detection of low-abundance targets.
Furthermore, this system is suitable for tissue multiplexing as multiple TSA dyes can be sequentially added through cyclical antibody stripping and re-probing.
Other benefits include up to 100x more sensitivity than indirect staining methods, achieving publication-quality data at the first attempt. A wide dynamic range enables the detection of low-abundance targets and easy multiplexing with no limitations on the primary antibody host.
For further information, contact United Bioresearch Products, the distributor of the full range of Proteintech products in Australia, or visit https://www.ptglab.com/products/tyramide-signaling-amplification-reagents/.
Phone: 02 4575 0309
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