Amsbio StemFit Basic05 medium for scalable hPSC manufacturing

Amsbio announces StemFit Basic05 — a next-generation, animal origin-free medium designed for scalable human pluripotent stem cell (hPSC) manufacturing.

Compatible with both 2D adherent and 3D suspension culture, the product enables a streamlined workflow from cell expansion to process scale-up. The use of a single medium across culture modes minimises the need for process redevelopment and optimisation traditionally associated with media changes. Pluripotent stem cells cultured in the medium also retain their differentiation capability and have been confirmed to be capable of differentiation directly from 3D aggregates.

For labs looking to implement stable and scalable suspension culture and scalable processes, high cell viability and uniform spheroid formation are essential. The medium has been demonstrated to deliver high cell viability and uniform spheroid formation across multiple human iPSC lines, along with maintenance of a normal karyotype.

Offering robust and highly reproducible uniform spheroid formation, the product is a useful process development medium that can reduce process complexity and development burden. It supports flexible culture scheduling, with comparable cell expansion even when weekend feeding is omitted.