Bio-Rad Laboratories anti-cemiplimab antibodies

Bio-Rad Laboratories has introduced a range of antibodies that are specific to cemiplimab (Libtayo) and inhibit the binding of the drug to its target, human programmed death receptor-1 (PD-1). The ready-made antibodies are suitable for developing selective and sensitive assays for bioanalysis and drug monitoring of cemiplimab.

Cemiplimab acts as a checkpoint inhibitor by binding to PD-1 on T cells and blocking the interaction with its ligands, PD-L1 and PD-L2, thereby activating T cells to attack cancer cells. The range of anti-cemiplimab antibodies offers optimal flexibility for bioanalytical ligand binding assays.

Three fully human IgG1 antibodies with varying levels of affinity are suitable for use as surrogate positive controls or calibrators in anti-drug antibody assays. These are complemented by a TrailBlazer Antibody with a SpyTag2 incorporated into its heavy chain, which enables site-directed conjugation or fast conversion to a bivalent Fab or an Ig-like format within an hour. Using the antibodies, a highly sensitive pharmacokinetic bridging ELISA can be developed to measure free drug.

The recombinant antibodies are generated using the Human Combinatorial Antibody Libraries (HuCAL) and CysDisplay, a method of phage display, along with guided selection methods to obtain highly targeted reagents. The recombinant production method results in batch-to-batch consistency, for reproducible results and a secure supply throughout the assay lifecycle.

The anti-cemiplimab antibodies are approved for in vitro research purposes, and for commercial applications providing in vitro testing services to support preclinical and clinical drug and biosimilar development.