Bio-Rad SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix reagent for qPCR data delivery

Bio-Rad Laboratories has launched the SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, a solution for obtaining reproducible, optimal-quality qPCR data from challenging samples.

Generating quality qPCR data from difficult-to-amplify samples - such as crude lysates from plants, tissues, formalin-fixed paraffin-embedded samples and other less-than-ideal sources that contain PCR inhibitors - can be challenging and frustrating. In these situations, PCR may be greatly inhibited, making it nearly impossible for researchers to obtain reliable data.

The product provides the advantages of other Bio-Rad supermixes with the added benefit of inhibitor tolerance. Its formulation has been optimised and validated to tolerate a wide spectrum of PCR inhibitors, including those found in crude lysates, polysaccharides and polyphenols, and various reagents left over from sample prep such as ethanol, isopropanol, EDTA and sodium chloride.

The inhibitor-tolerant version includes Bio-Rad’s Sso7d-fusion polymerase, which is engineered for enhanced qPCR performance. The supermix is compatible with all real-time PCR instruments and functions under any reaction condition.