BioLamina Laminin-521 cell culture matrix

Most established human pluripotent stem cell (hPSC) protocols require daily medium replacement, demanding researchers to routinely work weekends. BioLamina has developed a protocol that eliminates feeding on Saturdays and Sundays, based on the biorelevant support of Laminin-521 (LN-521). LN-521 is the key cell adhesion protein of the natural stem cell niche. It is expressed in the basement membranes of the developing embryo and secreted by hPSCs in culture.

The product means cells grow in a homogeneous monolayer without any need for manual removal of differentiated cell areas. Easy and efficient single-cell passaging enables standardised experiments. Cells also maintain high pluripotency expression and proliferation rate.

The protocol can easily be made totally defined and xeno-free with the user’s choice of culture medium and enzyme. The protocol is validated with a low bFGF concentration medium.

The stem cell matrix is available as a coating solution (LN-521) and as LN-521 pre-coated plates (521-To-Go).