BioStatus’s CyTRAK Orange is a novel fluorescent dye that preferentially stains the nucleus but also defines the cytoplasmic area in both live and fixed preparations. It is water-soluble and membrane-permeant and co-excites at 488 nm with eGFP, FITC and Cy2 fluorophores, avoiding the need for laser alignment in confocal instruments.
The product can be excited at 488 and 534 nm with optimal emission at 615 nm, with no UV/red excitation. It can be used in flow cytometry to separate (gate) nucleated cells without the time-consuming and potentially damaging RBC lysis procedures, and it does not crosslink with or esterify cytoplasmic proteins.
It is compatible with existing protocols and can be used across a wide range of instrumentation platforms: flow cytometers, epifluorescence and confocal microscopes, and image-based HCS platforms.
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