Biotage Isolute PLD+ protein and phospholipid removal plate

Isolute PLD+ is a protein and phospholipid removal plate for the clean-up of blood-based matrix samples for analysis by LC-MS/MS. The plates combine protein and phospholipid removal in a single product, providing effective and simple sample clean-up for LC-MS/MS analysis.

Utilising the company’s solvent crash/filter process, the plates also incorporate a phospholipid scavenging sorbent layer, which removes phospholipids from the sample during the filtration step. The plates remove more than 99% of plasma proteins and phospholipids, the main causes of ion suppression, leading to cleaner extracts and increased signal-to-noise sensitivity for a broad range of analytes.

Once purified, samples can be analysed directly or evaporated and reconstituted in a solvent that matches the user’s analytical method requirements. The plates can be processed using 96-well compatible positive pressure manifolds (eg, the Biotage Pressure+ 96), vacuum manifolds (eg, the Biotage VacMaster 96) and most automated liquid handling systems. The product is available in the SBS/ANSI standard 96-well plate format and typical sample volumes of 100 to 200 µL can be processed.