Enzo Life Sciences ROS-ID Total ROS/Superoxide detection kit

Enzo Life Sciences’ ROS-ID Total ROS/Superoxide detection kit includes dyes to measure reactive oxygen species (ROS) and superoxide production in live cells.

The non-fluorescent, cell-permeable Oxidative Stress Detection Reagent (green, Ex 490/525 nm) reacts directly with a wide range of reactive species (hydrogen peroxide, peroxynitrite and hydroxyl radicals), yielding a green fluorescent product. The Superoxide Detection Reagent (orange, Ex/Em 550/620 nm) is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. Pyocyanin (ROS inducer) and NAC (ROS inhibitor) are also provided with the kit.

The kit is compatible with major components of tissue culture media (phenol red, FBS and BSA) and has been validated on microscopy, flow cytometry and microplates. It is a member of the CELLESTIAL product line of reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications.