Epigentek EpiQuik MeDIP Ultra Kit for methylated DNA immunoprecipitation

The EpiQuik MeDIP Ultra Kit is a complete set of optimised reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format. The methylated DNA that is enriched with the kit can be used for various downstream applications, including qualitative and quantitative PCR (MeDIP-PCR), microarray (MeDIP-chip) and especially sequencing (MeDIP-seq).

The kit uses a monoclonal antibody, specific to 5-methylcytosine, to immunoprecipitate methylated genomic DNA. The antibody can strongly bind both single- and double-stranded DNA fragments containing two or more 5-mC, which enables highly sensitive enrichment of methylated DNA with >99% specificity. The highly sensitive and specific format of the kit can use DNA isolated from various species.

The kit adopts a fast and convenient protocol that can be completed in less than 3 h (from input sample to ready-to-use meDNA). Another advantage of the kit is that low DNA input is required - as low as 50 ng (10,000 cells) per reaction.