EpiGentek RNA Bisulfite Conversion Kit

The EpiGentek Methylamp RNA Bisulfite Conversion Kit is a complete set of optimised reagents required for fast bisulfite conversion on an RNA sample. Bisulfite conversion of RNA, followed by RT-PCR amplification, cloning and sequencing, yields information about RNA cytosine methylation states.

The converted RNA obtained with the kit is suitable for various downstream RNA methylation analyses including methylation specific RT-PCR, MS-HRM and bisulfite RNA-sequencing, for instance (pyrosequencing and deep-sequencing).

The kit includes a fast and convenient protocol that can be finished in 3 h. It completely converts unmethylated RNA cytosine into uracil (>99.9%) with no or negligible inappropriate/error conversion of methylcytosine to thymine (<0.1%) when the indicated range of input sample RNA is used.

The product offers powerful protection against RNA degradation, with over 90% of RNA loss prevented. Included control primers are specific against bisulfite-converted RNA and can be used to test whether the bisulfite conversion has been properly achieved.

A lot amount of input RNA can be used for bisulfite conversion, with as low as 5 ng per reaction. The kit ensures simple and consistent reaction conditions.