Hanging cell culture inserts

Hanging Millicell cell culture inserts are available pre-loaded into 24-well receiver trays. The pre-loaded assembly saves time and labour across a range of experimental protocols.

The inserts are plastic cell culture wells with membrane bottoms designed to promote more natural cell behaviour than plastic plates. The porous membrane allows cells to be fed (by adding nutrients to the medium) from both the apical and basolateral sides. As a result, the cells form high-integrity monolayers and studies are claimed to have shown that cell growth and function more closely resemble cells grown in vivo.

The hanging, pre-loaded inserts are available with a PET membrane in two pore sizes (0.4 and 8.0 µm). The units are sterile and the tissue culture is treated.

The inserts are suitable for co-culture and permeability assays including angiogenesis, chemotaxis, invasion, feeder layers, immunohistochemistry, tumour cell metastasis and invasion assays.

Inserts are also available as standing inserts or as 24-well or 96-well plate formats. They are available with a variety of microporous membranes, including PTFE, polycarbonate and mixed cellulose esters.