Vector Laboratories VectaFluor Duet immunofluorescence double-staining kit

Vector Laboratories introduces the VectaFluor Duet kit, offering convenient, ready-to-use immunofluorescence double labelling. The kit is suitable for use with a wide range of frozen and FFPE tissue samples, combining primary and secondary antibodies to streamline workflow.

The product includes a secondary reagent to detect mouse and rat primary antibodies with green and red DyLight fluorescent dyes and a normal blocking serum. While using two stains sequentially requires a tedious, time-consuming protocol and complex workflow, the VectaFluor Duet combines the double staining into one protocol. This reduces preparation time, according to the company — in some cases by as much as a day or more.

In the kit, Vector Laboratories’ affinity-purified, cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a process that ensures maximum labelling without compromising antibody affinity or specificity. The red and green DyLight anti-mouse/anti-rabbit antibodies are combined into a robust, stable cocktail that yields sensitive and consistent dual staining.