Wyatt Technology Möbius Computation of Protein Net Charge from Electrophoretic Mobility application note

Monday, 08 August, 2011 | Supplied by: Shimadzu Scientific Instruments (Oceania) Pty Ltd



Wyatt Technology’s Möbius electrophoretic mobility instrument can be used to measure precise protein charges. The optical design of the Möbius boosts the sensitivity of mobility measurements, enabling protein net charge characterisation at low concentrations. This capability is illustrated in an application note, ‘Möbius Computation of Protein Net Charge from Electrophoretic Mobility’, which is available to download free of charge via http://www.wyatt.com/literature/application-notes/mobius.html.

The application note describes how the Möbius overcomes the limitations of traditional phase analysis light scattering methods, eliminates undesirable interactions and facilitates protein charge measurements at low concentrations without damaging the protein itself. The Möbius mobility instrument is capable of carrying out protein net charge measurements with a moderate antibody concentration of 1.0 mg/mL.

The key to the successful measurement of a protein’s mobility lies in a much shortened measurement time and the availability of sufficient data to average away molecular diffusion. The Möbius achieves these goals through massive parallelism of its light scattering detection and extends the measurable molecular size range below 2 nm. A reduced measurement time of fewer than 60 s, in most cases, contributes to preservation of protein samples. The instrument also features a temperature control capability, making it suitable for performing automated temperature studies.

Online: www.shimadzu.com.au
Phone: 02 9684 4200
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