Instructions for Cryopreservation
Friday, 01 October, 2010
Introduction
Cryopreservation may compromise cell quality and performance. Performance of the cells CANNOT be guaranteed after cryopreservation. These instructions do not apply to Clonetics Hepatocytes, Neural Progenitor Cells, Poietics Adipose-Derived Stem Cells or Bovine Brain Microvascular Endothelial Cells.
Cryo freezing solutions used for Clonetics and Poietics products:
Clonetics Cells (see exceptions below)
80% Clonetics Growth Medium of Choice
10% FBS
10% DMSO
Mesenchymal Stem Cells
70% MSCBM
10% DMSO
20% Human Serum Albumin
Skeletal Muscle Cells
70% SkGM
20% FBS
10% DMSO
Poietics Cells
86.5% IMDM
7.5% DMSO
4% Human Serum Albumin
2% Hydroxy-ethyl-starch
General Instructions
1. Sterile filter freezing media using any 0.2 micron filter.
2. Harvest cells and centrifuge to collect cells into a pellet.
3. Resuspend cells in cold freezing solution at 500,000 to 2,000,000 cells per ml. Work quickly! Once exposed to DMSO, cells become very fragile.
4. Pipet aliquots (1 ml each) into freezing vials or ampoules and seal.
5. Insulate aliquots with Styrofoam or propanol freezing canister.
6. Store cells at -70°C overnight.
7. Within 12 to 24 hours, place in liquid Nitrogen for long-term storage (-200°C). Cells will be compromised by storage in -70°C.
Note: Lonza CANNOT guarantee performance of Clonetics and Poietics Cells that have been cryopreserved. To avoid loss of cells and forfeiture of your warranty, we recommend keeping cells in continuous culture without cryopreservation.
Nature-inspired filtration system recovers critical resources
A filtration system that can recover untapped critical resources such as copper and lithium from...
Optimising food and beverage testing with laboratory equipment
In the food and beverage industry, laboratories must balance precision, speed and compliance to...
Ocean acidification may be damaging sharks' teeth
Sharks can famously regrow their teeth — but this ability might not be enough to ensure...
