BD Rhapsody Single-Cell Analysis System

Wednesday, 01 December, 2021 | Supplied by: BD Life Science

BD Biosciences flow cytometry instruments have enabled researchers to conduct flow cytometry analysis for over 45 years. The BD Rhapsody System extends this capability and facilitates the analysis of thousands of genes and proteins simultaneously at the single-cell level. It can be used to access the vast wealth of information that exists at multiple levels in a cell to better understand biological networks that underlie phenotypic responses.

The system includes the BD Rhapsody Scanner and the BD Rhapsody Express Single-Cell Analysis System and allows high-throughput capture of multiomic information from single cells using a simple cartridge workflow and a multi-tier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries. NGS libraries are sequenced and analysed to provide high-dimensional resolution of single cells.

The BD Rhapsody Scanner is recommended when quality control is important, for users new to single-cell workflows, for the development of single-cell protocols and when working with novel cell types or complex cell systems. Scanner metrics can confirm quality of input cell sample and success of each step of the cartridge workflow; assess viability of cells until cells are lysed; and provide an estimate of the number of cells retrieved by sequencing. This information gives users the power to change course and troubleshoot experiments, if necessary, before expensive downstream sequencing.

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