Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing

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Clontech’s SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing enables sensitive mRNA-seq data from single cells and ultralow inputs. The kit provides direct cDNA synthesis from as few as 1-1000 intact cells (or as little as 10 pg-10 ng of total RNA), high reproducibility, gene body coverage and good representation of GC-rich transcripts.

The single-tube protocol works directly on whole cells and helps to preserve sample integrity. This is achieved via ligation-independent incorporation of adapters. The full-length cDNA libraries from the kit are compatible with both Illumina and Ion Torrent sequencing platforms.

The product is said to be capable of identifying the highest number of genes of any SMARTer Ultra Low kit, maintaining full-length gene information and a low percentage of rRNA reads. Its template switching efficiency is said to have been improved by building on LNA technology.

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